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cd138 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec cd138 microbeads
    Cd138 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd138+microbeads/CD138+MicroBeads%2C+human+-+lyophilized/pm42247288-208-5-7
    Average 96 stars, based on 44 article reviews
    cd138 microbeads - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Genome-wide copy number profiling enhances risk stratification in multiple myeloma by shallow whole-genome sequencing
    Article Snippet: .. Isolation of CD138-positive plasma cells was achieved within 48 hours of sample collection using CD138 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s protocol. ..

    Article Title: High diffuse bone marrow uptake in 18F-FDG PET/CT may reflect the diverse mutational characteristics of multiple myeloma.
    Article Snippet: .. CD138-positive MM cells were isolated from the BM samples using CD138 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. .. The isolated MM cells were subjected to targeted DNA sequencing using a customized panel developed by Macrogen (Seoul, Korea), based on NGS technology, which covered 80 genes that were potentially relevant to MM biology (Supplementary Table 1) [7].

    Article Title: Extracellular Vesicle IL5RA and BCMA in Serum Enable Non-Invasive Risk Stratification of Multiple Myeloma
    Article Snippet: .. CD138-positive plasma cells were isolated from bone marrow aspirates using magnetic-activated cell sorting (MACS) with an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, using CD138 microbeads (#130-105-961 and #130-051-301, Miltenyi Biotec). .. EVs were isolated from bone marrow supernatants and serum using a phosphatidylserine-binding affinity method with MagCaptureTM Exosome Isolation Kit PS Ver.

    Clinical Proteomics:

    Article Title: Genome-wide copy number profiling enhances risk stratification in multiple myeloma by shallow whole-genome sequencing
    Article Snippet: .. Isolation of CD138-positive plasma cells was achieved within 48 hours of sample collection using CD138 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s protocol. ..

    Article Title: Extracellular Vesicle IL5RA and BCMA in Serum Enable Non-Invasive Risk Stratification of Multiple Myeloma
    Article Snippet: .. CD138-positive plasma cells were isolated from bone marrow aspirates using magnetic-activated cell sorting (MACS) with an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, using CD138 microbeads (#130-105-961 and #130-051-301, Miltenyi Biotec). .. EVs were isolated from bone marrow supernatants and serum using a phosphatidylserine-binding affinity method with MagCaptureTM Exosome Isolation Kit PS Ver.

    FACS:

    Article Title: Extracellular Vesicle IL5RA and BCMA in Serum Enable Non-Invasive Risk Stratification of Multiple Myeloma
    Article Snippet: .. CD138-positive plasma cells were isolated from bone marrow aspirates using magnetic-activated cell sorting (MACS) with an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, using CD138 microbeads (#130-105-961 and #130-051-301, Miltenyi Biotec). .. EVs were isolated from bone marrow supernatants and serum using a phosphatidylserine-binding affinity method with MagCaptureTM Exosome Isolation Kit PS Ver.

    Article Title: Extracellular Vesicle IL5RA and BCMA in Serum Enable Non-Invasive Risk Stratification of Multiple Myeloma.
    Article Snippet: .. CD138-positive plasma cells were isolated from bone marrow aspirates using magnetic-activated cell sorting (MACS) with an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, using CD138 microbeads (#130-105-961 and #130-051-301, Miltenyi Biotec). .. EVs were isolated from bone marrow supernatants and serum using a phosphatidylserine-binding affinity method with MagCaptureTM Exosome Isolation Kit PS Ver.

    Magnetic Cell Separation:

    Article Title: Extracellular Vesicle IL5RA and BCMA in Serum Enable Non-Invasive Risk Stratification of Multiple Myeloma
    Article Snippet: .. CD138-positive plasma cells were isolated from bone marrow aspirates using magnetic-activated cell sorting (MACS) with an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, using CD138 microbeads (#130-105-961 and #130-051-301, Miltenyi Biotec). .. EVs were isolated from bone marrow supernatants and serum using a phosphatidylserine-binding affinity method with MagCaptureTM Exosome Isolation Kit PS Ver.

    Article Title: Extracellular Vesicle IL5RA and BCMA in Serum Enable Non-Invasive Risk Stratification of Multiple Myeloma.
    Article Snippet: .. CD138-positive plasma cells were isolated from bone marrow aspirates using magnetic-activated cell sorting (MACS) with an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, using CD138 microbeads (#130-105-961 and #130-051-301, Miltenyi Biotec). .. EVs were isolated from bone marrow supernatants and serum using a phosphatidylserine-binding affinity method with MagCaptureTM Exosome Isolation Kit PS Ver.

    Derivative Assay:

    Article Title: Decreased Expression of FAT4 Promotes Multiple Myeloma Proliferation and Migration by Targeting the Hippo/YAP Pathway.
    Article Snippet: .. CD138- positive cells were selected from bone marrow- derived mononuclear cells using CD138 MicroBeads (Miltenyi, Germany). .. The CD138+ cells were engaged in targeted gene sequencing performed by Righton Gene Co. Ltd. (China).

    Purification:

    Article Title: The C-terminal region of TENT5 proteins drives ER-associated mRNA polyadenylation via FNDC3 interaction.
    Article Snippet: LP1 were treated with Bortezomib (1 μM, Cell Signaling, 2204) or Bafilomycin A1 (Cayman Chemical, 11038). .. Human PCs were purified with CD138 microbeads (Miltenyi Biotec, 130-097-614) following manufacturer’s instructions. .. Primary murine PCs were obtained by LPS-stimulation of purified splenic B cells as previously described in.24 Briefly B cells were purified from total splenocytes by immunomagnetic negative selection (130-090-862; Miltenyi Biotec), after homogenization and 7 min of hypotonic lysis of red cells (ammonium chloride–potassium bicarbonate buffer) and were cultured in RPMI medium supplemented as described above and with 2-mercaptoethanol (50 μM).

    Labeling:

    Article Title: Comparative Analysis of Bone Marrow, cfDNA and CTCs for NGS-Based Multiple Myeloma Detection: A Pilot Study Indicating the Potential of CTCs
    Article Snippet: .. A small fraction was not enriched, but most cells were immunomagnetically labeled with CD138 Microbeads according to the manufacturer’s protocol (Miltenyi Biotec, Leiden, The Netherlands). .. Briefly, cells were washed and resuspended in running buffer (Miltenyi), with the addition of a blocking reagent (Miltenyi) and CD138 Microbeads (volumes were scaled to cell numbers).



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    (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), <t>CD138</t> (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).
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    Image Search Results


    Co-injection of human mast cells with primary CD138 MM cells leads to faster engraftment and bone destruction in the NSG-hIL6 MM PDX model.

    Journal: bioRxiv

    Article Title: Mast Cells Enhance Myeloma Engraftment and Promote Bone Destruction in the NSG-hIL6 Patient Derived Xenograft Model

    doi: 10.64898/2026.05.14.725220

    Figure Lengend Snippet: Co-injection of human mast cells with primary CD138 MM cells leads to faster engraftment and bone destruction in the NSG-hIL6 MM PDX model.

    Article Snippet: Fresh cells were subjected to CD138+ bead selection (Miltenyi 130-111-744) on LS columns (Miltenyi 30-042-401) according to manufacturer instructions and checked for purity by flow cytometry using singlet, live/dead (Thermo L10119) and intracellular light chain staining (Supplemental Figure 2).

    Techniques: Injection

    (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), CD138 (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).

    Journal: medRxiv

    Article Title: Single-Cell Analysis Reveals Inflammatory–Immunosuppressive Niches in Daratumumab-Resistant Primary AL Amyloidosis

    doi: 10.64898/2026.03.28.26349317

    Figure Lengend Snippet: (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), CD138 (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).

    Article Snippet: PC enrichment was performed using CD138 + Magnetic Beads (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat# 130-051-301), MS columns (Cat# 130-042-201), and a MiniMACS separator (Cat# 130-042-102).

    Techniques: Multiplex Assay, Immunofluorescence, Biomarker Discovery, Activation Assay